Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle
Ethanol-soluble proteins (EP) were obtained from spotless smoothhound (Mustelus griseus) muscle, and the EP antioxidant hydrolysates (E-AMH) were prepared using papain. Five antioxidant peptides were isolated from the E-AMH using ultrafiltration and consecutive chromatographic methods including ion-...
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doaj-71972c3e1abc44babc078cfa293a77872021-04-29T04:41:44ZengElsevierJournal of Functional Foods1756-46462014-01-016176185Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscleBin Wang0Yan-Dan Gong1Zhong-Rui Li2Di Yu3Chang-Feng Chi4Jian-Yin Ma5College of Biosystems Engineering and Food Science, Zhejiang University, 268 Kaixuan St., Hangzhou 310058, Zhejiang, PR China; School of Food and Pharmacy, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR China; Corresponding author at: 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR China. Tel.: +86 580 2555085; fax: +86 580 2554781.School of Food and Pharmacy, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR ChinaNational Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR ChinaSchool of Food and Pharmacy, Zhejiang Ocean University, 1 Ocean University S. Rd, Lincheng New District, Zhoushan 316022, PR ChinaEthanol-soluble proteins (EP) were obtained from spotless smoothhound (Mustelus griseus) muscle, and the EP antioxidant hydrolysates (E-AMH) were prepared using papain. Five antioxidant peptides were isolated from the E-AMH using ultrafiltration and consecutive chromatographic methods including ion-exchange chromatography, gel filtration chromatography, and RP-HPLC, and their sequences were identified as Gly-Ala-Ala (217.3 Da), Gly-Phe-Val-Gly (378.5 Da), Gly-Ile-Ile-Ser-His-Arg (682.0 Da), Glu-Leu-Leu-Ile (486.7 Da), and Lys-Phe-Pro-Glu (519.6 Da). All of these purified peptides exhibited high scavenging activity on hydroxyl radical (IC50 1.6337 mg/ml, 0.8944 mg/ml, 0.0769 mg/ml, 0.1173 mg/ml, and 0.1510 mg/ml, respectively), ABTS radical (IC50 1.7541 mg/ml, 1.3055 mg/ml, 0.3493 mg/ml, 0.3274 mg/ml and 0.4645 mg/ml, respectively), and superoxide radical (IC50 0.6714 mg/ml, 0.3082 mg/ml, 0.1508 mg/ml, 0.1851 mg/ml, and 0.2053 mg/ml, respectively). These five purified peptides also effectively inhibited the autooxidation in linoleic acid model system. The antioxidant activities of these purified peptides were attributed to their amino acid compositions, sequences, and hydrophobicities. These results suggested that these five peptides isolated from the E-AMH of spotless smoothhound muscle were potent antioxidants and might be effectively used as food additives and pharmaceutical agents.http://www.sciencedirect.com/science/article/pii/S1756464613002326Mustelus griseus (spotless smoothhound)Ethanol-soluble protein (EP)HydrolysateAntioxidant peptidesHydrophobic amino acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bin Wang Yan-Dan Gong Zhong-Rui Li Di Yu Chang-Feng Chi Jian-Yin Ma |
spellingShingle |
Bin Wang Yan-Dan Gong Zhong-Rui Li Di Yu Chang-Feng Chi Jian-Yin Ma Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle Journal of Functional Foods Mustelus griseus (spotless smoothhound) Ethanol-soluble protein (EP) Hydrolysate Antioxidant peptides Hydrophobic amino acid |
author_facet |
Bin Wang Yan-Dan Gong Zhong-Rui Li Di Yu Chang-Feng Chi Jian-Yin Ma |
author_sort |
Bin Wang |
title |
Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle |
title_short |
Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle |
title_full |
Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle |
title_fullStr |
Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle |
title_full_unstemmed |
Isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (Mustelus griseus) muscle |
title_sort |
isolation and characterisation of five novel antioxidant peptides from ethanol-soluble proteins hydrolysate of spotless smoothhound (mustelus griseus) muscle |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2014-01-01 |
description |
Ethanol-soluble proteins (EP) were obtained from spotless smoothhound (Mustelus griseus) muscle, and the EP antioxidant hydrolysates (E-AMH) were prepared using papain. Five antioxidant peptides were isolated from the E-AMH using ultrafiltration and consecutive chromatographic methods including ion-exchange chromatography, gel filtration chromatography, and RP-HPLC, and their sequences were identified as Gly-Ala-Ala (217.3 Da), Gly-Phe-Val-Gly (378.5 Da), Gly-Ile-Ile-Ser-His-Arg (682.0 Da), Glu-Leu-Leu-Ile (486.7 Da), and Lys-Phe-Pro-Glu (519.6 Da). All of these purified peptides exhibited high scavenging activity on hydroxyl radical (IC50 1.6337 mg/ml, 0.8944 mg/ml, 0.0769 mg/ml, 0.1173 mg/ml, and 0.1510 mg/ml, respectively), ABTS radical (IC50 1.7541 mg/ml, 1.3055 mg/ml, 0.3493 mg/ml, 0.3274 mg/ml and 0.4645 mg/ml, respectively), and superoxide radical (IC50 0.6714 mg/ml, 0.3082 mg/ml, 0.1508 mg/ml, 0.1851 mg/ml, and 0.2053 mg/ml, respectively). These five purified peptides also effectively inhibited the autooxidation in linoleic acid model system. The antioxidant activities of these purified peptides were attributed to their amino acid compositions, sequences, and hydrophobicities. These results suggested that these five peptides isolated from the E-AMH of spotless smoothhound muscle were potent antioxidants and might be effectively used as food additives and pharmaceutical agents. |
topic |
Mustelus griseus (spotless smoothhound) Ethanol-soluble protein (EP) Hydrolysate Antioxidant peptides Hydrophobic amino acid |
url |
http://www.sciencedirect.com/science/article/pii/S1756464613002326 |
work_keys_str_mv |
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