Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men

Appetite loss is a common symptom that occurs in high altitude (HA) for lowlanders. Previous studies indicated that hypoxia is the initiating vital factor of HA appetite loss. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 play important roles in hypoxic responses. We aimed to explore the associatio...

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Main Authors: Wenxu Pan, Chuan Liu, Jihang Zhang, Xubin Gao, Shiyong Yu, Hu Tan, Jie Yu, Dehui Qian, Jiabei Li, Shizhu Bian, Jie Yang, Chen Zhang, Lan Huang, Jun Jin
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Physiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2019.00059/full
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language English
format Article
sources DOAJ
author Wenxu Pan
Chuan Liu
Jihang Zhang
Xubin Gao
Shiyong Yu
Shiyong Yu
Hu Tan
Hu Tan
Jie Yu
Jie Yu
Dehui Qian
Dehui Qian
Jiabei Li
Jiabei Li
Shizhu Bian
Shizhu Bian
Jie Yang
Chen Zhang
Lan Huang
Lan Huang
Jun Jin
Jun Jin
spellingShingle Wenxu Pan
Chuan Liu
Jihang Zhang
Xubin Gao
Shiyong Yu
Shiyong Yu
Hu Tan
Hu Tan
Jie Yu
Jie Yu
Dehui Qian
Dehui Qian
Jiabei Li
Jiabei Li
Shizhu Bian
Shizhu Bian
Jie Yang
Chen Zhang
Lan Huang
Lan Huang
Jun Jin
Jun Jin
Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
Frontiers in Physiology
high altitude
appetite loss
hypoxia
PPARA
EPAS1
single nucleotide polymorphism
author_facet Wenxu Pan
Chuan Liu
Jihang Zhang
Xubin Gao
Shiyong Yu
Shiyong Yu
Hu Tan
Hu Tan
Jie Yu
Jie Yu
Dehui Qian
Dehui Qian
Jiabei Li
Jiabei Li
Shizhu Bian
Shizhu Bian
Jie Yang
Chen Zhang
Lan Huang
Lan Huang
Jun Jin
Jun Jin
author_sort Wenxu Pan
title Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
title_short Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
title_full Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
title_fullStr Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
title_full_unstemmed Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young Men
title_sort association between single nucleotide polymorphisms in ppara and epas1 genes and high-altitude appetite loss in chinese young men
publisher Frontiers Media S.A.
series Frontiers in Physiology
issn 1664-042X
publishDate 2019-02-01
description Appetite loss is a common symptom that occurs in high altitude (HA) for lowlanders. Previous studies indicated that hypoxia is the initiating vital factor of HA appetite loss. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 play important roles in hypoxic responses. We aimed to explore the association of these hypoxia-related gene polymorphisms with HA appetite loss. In this study, we enrolled 416 young men who rapidly ascended to Lhasa (3700 m) from Chengdu (<500m) by plane. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 were genotyped by MassARRAY. Appetite scores were measured to identify HA appetite loss. Logistic regression and multiple genetic models were tested to evaluate the association between the single nucleotide polymorphisms (SNPs) and risk of HA appetite loss in crude and adjusted (age and SaO2) analysis. Subsequently, Haploview software was used to analyze the linkage disequilibrium (LD), haplotype construction and the association of diverse haplotypes with the risk of HA appetite loss. Our results revealed that allele “A” in PPARA rs4253747 was significantly associated with the increased risk of HA appetite loss. Codominant, dominant, recessive, and log-additive models of PPARA rs4253747 showed the increased risk of HA appetite loss in the crude and adjusted analysis. However, only dominant, overdominant, and log-additive models of EPAS1 rs6756667 showed decreased risk of HA appetite loss in the crude and adjusted analysis. Moreover, the results from haplotype-based test showed that the rs7292407-rs6520015 haplotype “AC” was associated with HA appetite loss in the crude analysis rather than the adjusted analysis. In this study, we first established the association of SNPs in PPARA (rs4253747) and EPAS1 (rs6756667) genes with susceptibility to HA appetite loss in Han Chinese young men. These findings provide novel insights into understanding the mechanisms involved in HA appetite loss.
topic high altitude
appetite loss
hypoxia
PPARA
EPAS1
single nucleotide polymorphism
url https://www.frontiersin.org/article/10.3389/fphys.2019.00059/full
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spelling doaj-7199e7d0253a4f6f98f4a6cb27080b1d2020-11-24T23:52:45ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-02-011010.3389/fphys.2019.00059434174Association Between Single Nucleotide Polymorphisms in PPARA and EPAS1 Genes and High-Altitude Appetite Loss in Chinese Young MenWenxu Pan0Chuan Liu1Jihang Zhang2Xubin Gao3Shiyong Yu4Shiyong Yu5Hu Tan6Hu Tan7Jie Yu8Jie Yu9Dehui Qian10Dehui Qian11Jiabei Li12Jiabei Li13Shizhu Bian14Shizhu Bian15Jie Yang16Chen Zhang17Lan Huang18Lan Huang19Jun Jin20Jun Jin21Department of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaDepartment of Cardiology, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaInstitute of Cardiovascular Diseases, Xinqiao Hospital, Army Medical University (The Third Military Medical University), Chongqing, ChinaAppetite loss is a common symptom that occurs in high altitude (HA) for lowlanders. Previous studies indicated that hypoxia is the initiating vital factor of HA appetite loss. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 play important roles in hypoxic responses. We aimed to explore the association of these hypoxia-related gene polymorphisms with HA appetite loss. In this study, we enrolled 416 young men who rapidly ascended to Lhasa (3700 m) from Chengdu (<500m) by plane. PPARA, EPAS1, EGLN1, HIF1A, HIF1AN, and NFE2L2 were genotyped by MassARRAY. Appetite scores were measured to identify HA appetite loss. Logistic regression and multiple genetic models were tested to evaluate the association between the single nucleotide polymorphisms (SNPs) and risk of HA appetite loss in crude and adjusted (age and SaO2) analysis. Subsequently, Haploview software was used to analyze the linkage disequilibrium (LD), haplotype construction and the association of diverse haplotypes with the risk of HA appetite loss. Our results revealed that allele “A” in PPARA rs4253747 was significantly associated with the increased risk of HA appetite loss. Codominant, dominant, recessive, and log-additive models of PPARA rs4253747 showed the increased risk of HA appetite loss in the crude and adjusted analysis. However, only dominant, overdominant, and log-additive models of EPAS1 rs6756667 showed decreased risk of HA appetite loss in the crude and adjusted analysis. Moreover, the results from haplotype-based test showed that the rs7292407-rs6520015 haplotype “AC” was associated with HA appetite loss in the crude analysis rather than the adjusted analysis. In this study, we first established the association of SNPs in PPARA (rs4253747) and EPAS1 (rs6756667) genes with susceptibility to HA appetite loss in Han Chinese young men. These findings provide novel insights into understanding the mechanisms involved in HA appetite loss.https://www.frontiersin.org/article/10.3389/fphys.2019.00059/fullhigh altitudeappetite losshypoxiaPPARAEPAS1single nucleotide polymorphism