Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain

Expression, purification and characterization of cellobiose dehydrogenase mutants from Phanerochaete chrysosporium in Pichia pastoris KM71H strain

Production of soluble cellobiose dehydrogenase (CDH) mutant proteins previously evolved on the surface of S. cerevisiae yeast cells was established for use in biosensors and biofuel cells. For this purpose, mutant cdh genes tm (D20N, A64T, V592M), H5 (D20N, V22A, A64T, V592M) and H9 (D20N, A64T, T84...

Full description

Bibliographic Details
Main Authors: Balaž Ana Marija J., Blažić Marija B., Popović Nikolina, Prodanović Olivera L., Ostafe Raluca V., Fischer Rainer, Prodanović Radivoje M.
Format: Article
Language:English
Published: Serbian Chemical Society 2020-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
mutant proteins
yeast
protein purification
kinetic characterization
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51391900058B.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0352-5139/2020/0352-51391900058B.pdf

Similar Items