Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts
The pathophysiological response to a severe burn injury involves a robust increase in circulating inflammatory/endocrine factors and a hypermetabolic state, both of which contribute to prolonged skeletal muscle atrophy. In order to characterize the role of circulating factors in muscle atrophy follo...
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doaj-727536f8cbb04bb5bbc0b1a9365fd8d62020-11-24T21:40:13ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2015-06-01610.3389/fphys.2015.00184135810Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblastsKatie L Corrick0Michael J Stec1Michael J Stec2Edward K Merritt3Edward K Merritt4Samuel T Windham5Samuel T Windham6Steven J Thomas7James M Cross8Marcas M Bamman9Marcas M Bamman10Marcas M Bamman11University of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamUniversity of Texas Health Science CenterUniversity of Alabama at BirminghamUniversity of Alabama at BirminghamBirmingham VA Medical CenterThe pathophysiological response to a severe burn injury involves a robust increase in circulating inflammatory/endocrine factors and a hypermetabolic state, both of which contribute to prolonged skeletal muscle atrophy. In order to characterize the role of circulating factors in muscle atrophy following a burn injury, human skeletal muscle satellite cells were grown in culture and differentiated to myoblasts/myotubes in media containing serum from burn patients or healthy, age and sex-matched controls. While incubation in burn serum did not affect NFκB signaling, cells incubated in burn serum displayed a transient increase in STAT3 phosphorlyation (Tyr705) after 48h of treatment with burn serum (≈+70%; P<0.01), with these levels returning to normal by 96h. Muscle cells differentiated in burn serum displayed reduced myogenic fusion signaling (phospho-STAT6 (Tyr641), ≈-75%; ADAM12, ≈-20%; both P<0.01), and reduced levels of myogenin (≈-75%; P<0.05). Concomitantly, myotubes differentiated in burn serum demonstrated impaired myogenesis (assessed by number of nuclei/myotube). Incubation in burn serum for 96h did not increase proteolytic signaling (assessed via caspase-3 and ubiquitin levels), but reduced anabolic signaling (p-p70S6k (Ser421/Thr424), -30%; p-rpS6 (Ser240/244), ≈-50%) and impaired protein synthesis (-24%) (P<0.05). This resulted in a loss of total protein content (-18%) and reduced cell size (-33%) (P<0.05). Overall, incubation of human muscle cells in serum from burn patients results in impaired myogenesis and reduced myotube size, indicating that circulating factors may play a significant role in muscle loss and impaired muscle recovery following burn injury.http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00184/fullInflammationmyogenesisburnMuscle protein synthesisMyoblastMyotube |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katie L Corrick Michael J Stec Michael J Stec Edward K Merritt Edward K Merritt Samuel T Windham Samuel T Windham Steven J Thomas James M Cross Marcas M Bamman Marcas M Bamman Marcas M Bamman |
spellingShingle |
Katie L Corrick Michael J Stec Michael J Stec Edward K Merritt Edward K Merritt Samuel T Windham Samuel T Windham Steven J Thomas James M Cross Marcas M Bamman Marcas M Bamman Marcas M Bamman Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts Frontiers in Physiology Inflammation myogenesis burn Muscle protein synthesis Myoblast Myotube |
author_facet |
Katie L Corrick Michael J Stec Michael J Stec Edward K Merritt Edward K Merritt Samuel T Windham Samuel T Windham Steven J Thomas James M Cross Marcas M Bamman Marcas M Bamman Marcas M Bamman |
author_sort |
Katie L Corrick |
title |
Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
title_short |
Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
title_full |
Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
title_fullStr |
Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
title_full_unstemmed |
Serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
title_sort |
serum from human burn victims impairs myogenesis and protein synthesis in primary myoblasts |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2015-06-01 |
description |
The pathophysiological response to a severe burn injury involves a robust increase in circulating inflammatory/endocrine factors and a hypermetabolic state, both of which contribute to prolonged skeletal muscle atrophy. In order to characterize the role of circulating factors in muscle atrophy following a burn injury, human skeletal muscle satellite cells were grown in culture and differentiated to myoblasts/myotubes in media containing serum from burn patients or healthy, age and sex-matched controls. While incubation in burn serum did not affect NFκB signaling, cells incubated in burn serum displayed a transient increase in STAT3 phosphorlyation (Tyr705) after 48h of treatment with burn serum (≈+70%; P<0.01), with these levels returning to normal by 96h. Muscle cells differentiated in burn serum displayed reduced myogenic fusion signaling (phospho-STAT6 (Tyr641), ≈-75%; ADAM12, ≈-20%; both P<0.01), and reduced levels of myogenin (≈-75%; P<0.05). Concomitantly, myotubes differentiated in burn serum demonstrated impaired myogenesis (assessed by number of nuclei/myotube). Incubation in burn serum for 96h did not increase proteolytic signaling (assessed via caspase-3 and ubiquitin levels), but reduced anabolic signaling (p-p70S6k (Ser421/Thr424), -30%; p-rpS6 (Ser240/244), ≈-50%) and impaired protein synthesis (-24%) (P<0.05). This resulted in a loss of total protein content (-18%) and reduced cell size (-33%) (P<0.05). Overall, incubation of human muscle cells in serum from burn patients results in impaired myogenesis and reduced myotube size, indicating that circulating factors may play a significant role in muscle loss and impaired muscle recovery following burn injury. |
topic |
Inflammation myogenesis burn Muscle protein synthesis Myoblast Myotube |
url |
http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00184/full |
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