Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways
Summary: Understanding the mechanisms for cellular aging is a fundamental question in biology. Normal red blood cells (RBCs) survive for approximately 100 days, and their survival is likely limited by functional decline secondary to cumulative damage to cell constituents, which may be reflected in a...
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2020-10-01
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doaj-728872d5a239437587e435d3af7420202020-11-25T03:37:09ZengElsevieriScience2589-00422020-10-012310101630Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic PathwaysNeema Jamshidi0Xiuling Xu1Katharina von Löhneysen2Katrin Soldau3Rob P. Mohney4Edward D. Karoly5Mike Scott6Jeffrey S. Friedman7University of California, San Diego, Institute of Engineering in Medicine, La Jolla, CA, USA; University of California, Los Angeles, Department of Radiological Sciences, Los Angeles, CA, USAThe Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, CA, USAMitokinin, Inc, San Francisco, CA, USAUniversity of California, San Diego, Department of Pathology, La Jolla, CA, USAMetabolon, Inc, Durham, NC, USAMetabolon, Inc, Durham, NC, USASan Diego Mesa College, Chemistry Department, San Diego, CA, USAFriedman Bioventure, Inc, San Diego, CA, USA; DTx Pharma, Inc, San Diego, CA, USA; Corresponding authorSummary: Understanding the mechanisms for cellular aging is a fundamental question in biology. Normal red blood cells (RBCs) survive for approximately 100 days, and their survival is likely limited by functional decline secondary to cumulative damage to cell constituents, which may be reflected in altered metabolic capabilities. To investigate metabolic changes during in vivo RBC aging, labeled cell populations were purified at intervals and assessed for abundance of metabolic intermediates using mass spectrometry. A total of 167 metabolites were profiled and quantified from cell populations of defined ages. Older RBCs maintained ATP and redox charge states at the cost of altered activity of enzymatic pathways. Time-dependent changes were identified in metabolites related to maintenance of the redox state and membrane structure. These findings illuminate the differential metabolic pathway usage associated with normal cellular aging and identify potential biomarkers to determine average RBC age and rates of RBC turnover from a single blood sample.http://www.sciencedirect.com/science/article/pii/S2589004220308221Medical BiochemistryMetabolomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neema Jamshidi Xiuling Xu Katharina von Löhneysen Katrin Soldau Rob P. Mohney Edward D. Karoly Mike Scott Jeffrey S. Friedman |
spellingShingle |
Neema Jamshidi Xiuling Xu Katharina von Löhneysen Katrin Soldau Rob P. Mohney Edward D. Karoly Mike Scott Jeffrey S. Friedman Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways iScience Medical Biochemistry Metabolomics |
author_facet |
Neema Jamshidi Xiuling Xu Katharina von Löhneysen Katrin Soldau Rob P. Mohney Edward D. Karoly Mike Scott Jeffrey S. Friedman |
author_sort |
Neema Jamshidi |
title |
Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways |
title_short |
Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways |
title_full |
Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways |
title_fullStr |
Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways |
title_full_unstemmed |
Metabolome Changes during In Vivo Red Cell Aging Reveal Disruption of Key Metabolic Pathways |
title_sort |
metabolome changes during in vivo red cell aging reveal disruption of key metabolic pathways |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2020-10-01 |
description |
Summary: Understanding the mechanisms for cellular aging is a fundamental question in biology. Normal red blood cells (RBCs) survive for approximately 100 days, and their survival is likely limited by functional decline secondary to cumulative damage to cell constituents, which may be reflected in altered metabolic capabilities. To investigate metabolic changes during in vivo RBC aging, labeled cell populations were purified at intervals and assessed for abundance of metabolic intermediates using mass spectrometry. A total of 167 metabolites were profiled and quantified from cell populations of defined ages. Older RBCs maintained ATP and redox charge states at the cost of altered activity of enzymatic pathways. Time-dependent changes were identified in metabolites related to maintenance of the redox state and membrane structure. These findings illuminate the differential metabolic pathway usage associated with normal cellular aging and identify potential biomarkers to determine average RBC age and rates of RBC turnover from a single blood sample. |
topic |
Medical Biochemistry Metabolomics |
url |
http://www.sciencedirect.com/science/article/pii/S2589004220308221 |
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1724546883718742016 |