LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells
Abstract Background The effect of lncRNA FTX on non-alcoholic fatty liver disease (NAFLD) conversion to hepatocellular carcinoma (HCC) is unclear. Methods In our study, C57BL/6 mice was fed with high fat diet for obtaining NAFLD mouse model, and diethylnitrosamine induced the formation of HCC tumor....
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doaj-7314724a77484499be998aa2cb89f6552020-11-25T02:44:19ZengBMCCancer Cell International1475-28672020-06-0120111110.1186/s12935-020-01354-0LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cellsHuajun Wu0Zhiwei Zhong1Anji Wang2Chunhui Yuan3Ke Ning4Huanhuan Hu5Chao Wang6Xiangbao Yin7Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital of Nanchang UniversityAbstract Background The effect of lncRNA FTX on non-alcoholic fatty liver disease (NAFLD) conversion to hepatocellular carcinoma (HCC) is unclear. Methods In our study, C57BL/6 mice was fed with high fat diet for obtaining NAFLD mouse model, and diethylnitrosamine induced the formation of HCC tumor. The expression of iNOS and CD206 in tissues were examined using immunohistochemistry. In addition, qRT-PCR was implemented to detect the expression of FTX and mRNAs. The percentage of M1 and M2 Kupffer cells (KCs) were determined using flow cytometry. The pathological change in liver tissues was displayed by H&E staining. Besides, immunofluorescence assay was performed to ensure the primary KCs through labeling F4/80. Results Here, we found that the expression of FTX and the ratio of M1/M2 KCs in liver tissues from NAFLD-transformed HCC (NAFLD-HCC) patients lower than in liver tissues from NAFLD patients. Subsequently, we revealed that the expression of FTX and M1/M2 KCs ratio were downregulated during NAFLD conversion to HCC. Importantly, increasing of FTX inhibited HCC tumor growth, improved liver damage and promoted M1 polarization of KCs during NAFLD conversion to HCC, while these effects of FTX were reversed by inactivating of KCs. Finally, in vitro experiments, our data indicated that FTX facilitated the M1 polarization of KCs. Conclusion In conclusion, our results demonstrated that upregulation of FTX suppressed NAFLD conversion to HCC though promoting M1 polarization of KCs. Our findings presented a new regulatory mechanism for NAFLD conversion to HCC, and provided a new biomarker for inhibiting this conversion.http://link.springer.com/article/10.1186/s12935-020-01354-0Hepatocellular carcinomalncRNA FTXNon-alcoholic fatty liver diseaseKupffer cell |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huajun Wu Zhiwei Zhong Anji Wang Chunhui Yuan Ke Ning Huanhuan Hu Chao Wang Xiangbao Yin |
spellingShingle |
Huajun Wu Zhiwei Zhong Anji Wang Chunhui Yuan Ke Ning Huanhuan Hu Chao Wang Xiangbao Yin LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells Cancer Cell International Hepatocellular carcinoma lncRNA FTX Non-alcoholic fatty liver disease Kupffer cell |
author_facet |
Huajun Wu Zhiwei Zhong Anji Wang Chunhui Yuan Ke Ning Huanhuan Hu Chao Wang Xiangbao Yin |
author_sort |
Huajun Wu |
title |
LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells |
title_short |
LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells |
title_full |
LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells |
title_fullStr |
LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells |
title_full_unstemmed |
LncRNA FTX represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the M1/M2 polarization of Kupffer cells |
title_sort |
lncrna ftx represses the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via regulating the m1/m2 polarization of kupffer cells |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2020-06-01 |
description |
Abstract Background The effect of lncRNA FTX on non-alcoholic fatty liver disease (NAFLD) conversion to hepatocellular carcinoma (HCC) is unclear. Methods In our study, C57BL/6 mice was fed with high fat diet for obtaining NAFLD mouse model, and diethylnitrosamine induced the formation of HCC tumor. The expression of iNOS and CD206 in tissues were examined using immunohistochemistry. In addition, qRT-PCR was implemented to detect the expression of FTX and mRNAs. The percentage of M1 and M2 Kupffer cells (KCs) were determined using flow cytometry. The pathological change in liver tissues was displayed by H&E staining. Besides, immunofluorescence assay was performed to ensure the primary KCs through labeling F4/80. Results Here, we found that the expression of FTX and the ratio of M1/M2 KCs in liver tissues from NAFLD-transformed HCC (NAFLD-HCC) patients lower than in liver tissues from NAFLD patients. Subsequently, we revealed that the expression of FTX and M1/M2 KCs ratio were downregulated during NAFLD conversion to HCC. Importantly, increasing of FTX inhibited HCC tumor growth, improved liver damage and promoted M1 polarization of KCs during NAFLD conversion to HCC, while these effects of FTX were reversed by inactivating of KCs. Finally, in vitro experiments, our data indicated that FTX facilitated the M1 polarization of KCs. Conclusion In conclusion, our results demonstrated that upregulation of FTX suppressed NAFLD conversion to HCC though promoting M1 polarization of KCs. Our findings presented a new regulatory mechanism for NAFLD conversion to HCC, and provided a new biomarker for inhibiting this conversion. |
topic |
Hepatocellular carcinoma lncRNA FTX Non-alcoholic fatty liver disease Kupffer cell |
url |
http://link.springer.com/article/10.1186/s12935-020-01354-0 |
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