Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues

Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues

Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence the evolutionary trajectory, an aspect that is often overlooked in laboratory directed evolution studies. We selected the Escherichia coli nit...

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Bibliographic Details
Main Authors: Kelsi R Hall, Katherine J Robins, Elsie M Williams, Michelle H Rich, Mark J Calcott, Janine N Copp, Rory F Little, Ralf Schwörer, Gary B Evans, Wayne M Patrick, David F Ackerley
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-11-01
Series:eLife
Subjects:
nitroreductase
enzyme promiscuity
substrate competition
directed evolution
enzyme evolution
Online Access:https://elifesciences.org/articles/59081

Internet

https://elifesciences.org/articles/59081

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