Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template [v1; ref status: indexed, http://f1000r.es/4sm]

Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-s...

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Bibliographic Details
Main Authors: Vikash Bhardwaj, Kulbhushan Sharma
Format: Article
Language:English
Published: F1000 Research Ltd 2014-12-01
Series:F1000Research
Subjects:
Online Access:http://f1000research.com/articles/3-320/v1