Recombinant Lectin from Tepary Bean (<i>Phaseolus acutifolius</i>) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification

Recombinant Lectin from Tepary Bean (<i>Phaseolus acutifolius</i>) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification

Herein, we report the production of a recombinant Tepary bean lectin (<i>r</i>TBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates. <i>r</i>TBL-1 was expressed in <i>Pichia pastoris,</i> yielding 316 mg per...

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Bibliographic Details
Main Authors: Dania Martínez-Alarcón, Annabelle Varrot, Elaine Fitches, John A. Gatehouse, Min Cao, Prashant Pyati, Alejandro Blanco-Labra, Teresa Garcia-Gasca
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Biomolecules
Subjects:
recombinant lectins
Tepary bean
<i>Pichia pastoris</i>
MGAT5
glycan array
structure
Online Access:https://www.mdpi.com/2218-273X/10/4/654

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https://www.mdpi.com/2218-273X/10/4/654

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