Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology

Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology

A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a...

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Bibliographic Details
Main Authors: Diego Sebastián Insuasty Cepeda, Héctor Manuel Pineda Castañeda, Andrea Verónica Rodríguez Mayor, Javier Eduardo García Castañeda, Mauricio Maldonado Villamil, Ricardo Fierro Medina, Zuly Jenny Rivera Monroy
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Molecules
Subjects:
peptide
solid phase extraction (SPE)
preparative purification
gradient elution
solid phase peptide synthesis
Online Access:https://www.mdpi.com/1420-3049/24/7/1215

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https://www.mdpi.com/1420-3049/24/7/1215

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