Trycycler: consensus long-read assemblies for bacterial genomes

• Main Authors: Ryan R. Wick, Louise M. Judd, Louise T. Cerdeira, Jane Hawkey, Guillaume Méric, Ben Vezina, Kelly L. Wyres, Kathryn E. Holt
• Format: Article
• Language: English
• Published: BMC 2021-09-01
• Series: Genome Biology
• Subjects: Genome assembly; Bacterial genomics; Whole-genome sequencing; Long-read sequencing; Oxford Nanopore sequencing
• Online Access: https://doi.org/10.1186/s13059-021-02483-z

Abstract While long-read sequencing allows for the complete assembly of bacterial genomes, long-read assemblies contain a variety of errors. Here, we present Trycycler, a tool which produces a consensus assembly from multiple input assemblies of the same genome. Benchmarking showed that Trycycler assemblies contained fewer errors than assemblies constructed with a single tool. Post-assembly polishing further reduced errors and Trycycler+polishing assemblies were the most accurate genomes in our study. As Trycycler requires manual intervention, its output is not deterministic. However, we demonstrated that multiple users converge on similar assemblies that are consistently more accurate than those produced by automated assembly tools.