HaloTag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors

The production of recombinant proteins such as the fibroblast growth factors (FGFs) is the key to establishing their function in cell communication. The production of recombinant FGFs in E. coli is limited, however, due to expression and solubility problems. HaloTag has been used as a fusion protein...

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Bibliographic Details
Main Authors: Changye Sun, Yong Li, Sarah E. Taylor, Xianqing Mao, Mark C. Wilkinson, David G. Fernig
Format: Article
Language:English
Published: PeerJ Inc. 2015-06-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/1060.pdf