Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin
A 9-cis-epoxycarotenoid dioxygenase gene from Serratia sp. ATCC 39,006 (SeNCED) was overexpressed in soluble form in E.coli. SeNCED showed the maximum activity at 30 °C and pH 8.0, and it was stable relatively at range of pH 5–10 and temperature of 20 °C to 30 °C. SeNCED effectively catalyzes the si...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2018-06-01
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Series: | Biotechnology Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X18300894 |