pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers
For improving the therapeutic efficiency of tumors and decreasing undesirable side effects, ternary complexes were developed by coating pH-sensitive PEG-b-PLL-g-succinylsulfathiazole (hereafter abbreviated as PPSD) with DNA/PEI polyplexes via electrostatic interaction. PPSD can efficiently shield th...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2016-04-01
|
Series: | Polymers |
Subjects: | |
Online Access: | http://www.mdpi.com/2073-4360/8/4/141 |
id |
doaj-749034ce6bc046598a7dc99a03769d04 |
---|---|
record_format |
Article |
spelling |
doaj-749034ce6bc046598a7dc99a03769d042020-11-24T21:07:23ZengMDPI AGPolymers2073-43602016-04-018414110.3390/polym8040141polym8040141pH-Triggered Sheddable Shielding System for Polycationic Gene CarriersJialiang Xia0Huayu Tian1Jie Chen2Lin Lin3Zhaopei Guo4Bing Han5Hongyan Yang6Zongcai Feng7School of Chemistry and Chemical Engineering, Lingnan Normal University, 29 Cunjin Road, Zhanjiang 524048, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 5625 Renmin Street, Changchun 130022, ChinaSchool of Chemistry and Chemical Engineering, Lingnan Normal University, 29 Cunjin Road, Zhanjiang 524048, ChinaSchool of Chemistry and Chemical Engineering, Lingnan Normal University, 29 Cunjin Road, Zhanjiang 524048, ChinaSchool of Chemistry and Chemical Engineering, Lingnan Normal University, 29 Cunjin Road, Zhanjiang 524048, ChinaFor improving the therapeutic efficiency of tumors and decreasing undesirable side effects, ternary complexes were developed by coating pH-sensitive PEG-b-PLL-g-succinylsulfathiazole (hereafter abbreviated as PPSD) with DNA/PEI polyplexes via electrostatic interaction. PPSD can efficiently shield the surface charge of DNA/PEI. The gene transfection efficiency of ternary complexes was lower than that of DNA/PEI at pH 7.4; however, it recovered to the same level as that of DNA/PEI at pH 6.0, attributed to the pH-triggered release of DNA/PEI from ternary complexes. Cell uptake results also exhibited the same trend as transfection at different pH values. The suitable ability for pH-triggered shielding/deshielding estimated that PPSD demonstrates potential as a shielding system for use in in vivo gene delivery.http://www.mdpi.com/2073-4360/8/4/141gene deliveryshielding systempH-sensitive |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jialiang Xia Huayu Tian Jie Chen Lin Lin Zhaopei Guo Bing Han Hongyan Yang Zongcai Feng |
spellingShingle |
Jialiang Xia Huayu Tian Jie Chen Lin Lin Zhaopei Guo Bing Han Hongyan Yang Zongcai Feng pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers Polymers gene delivery shielding system pH-sensitive |
author_facet |
Jialiang Xia Huayu Tian Jie Chen Lin Lin Zhaopei Guo Bing Han Hongyan Yang Zongcai Feng |
author_sort |
Jialiang Xia |
title |
pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers |
title_short |
pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers |
title_full |
pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers |
title_fullStr |
pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers |
title_full_unstemmed |
pH-Triggered Sheddable Shielding System for Polycationic Gene Carriers |
title_sort |
ph-triggered sheddable shielding system for polycationic gene carriers |
publisher |
MDPI AG |
series |
Polymers |
issn |
2073-4360 |
publishDate |
2016-04-01 |
description |
For improving the therapeutic efficiency of tumors and decreasing undesirable side effects, ternary complexes were developed by coating pH-sensitive PEG-b-PLL-g-succinylsulfathiazole (hereafter abbreviated as PPSD) with DNA/PEI polyplexes via electrostatic interaction. PPSD can efficiently shield the surface charge of DNA/PEI. The gene transfection efficiency of ternary complexes was lower than that of DNA/PEI at pH 7.4; however, it recovered to the same level as that of DNA/PEI at pH 6.0, attributed to the pH-triggered release of DNA/PEI from ternary complexes. Cell uptake results also exhibited the same trend as transfection at different pH values. The suitable ability for pH-triggered shielding/deshielding estimated that PPSD demonstrates potential as a shielding system for use in in vivo gene delivery. |
topic |
gene delivery shielding system pH-sensitive |
url |
http://www.mdpi.com/2073-4360/8/4/141 |
work_keys_str_mv |
AT jialiangxia phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT huayutian phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT jiechen phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT linlin phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT zhaopeiguo phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT binghan phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT hongyanyang phtriggeredsheddableshieldingsystemforpolycationicgenecarriers AT zongcaifeng phtriggeredsheddableshieldingsystemforpolycationicgenecarriers |
_version_ |
1716763039165841408 |