Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium
The electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The...
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doaj-74b930496c6c4b049e73ef0d1f8cd97d2020-11-24T22:57:05ZengElsevierJournal of Pharmacological Sciences1347-86132017-04-01133419520210.1016/j.jphs.2016.12.006Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardiumYayoi Tsuneoka0Masahiko Irie1Yusuke Tanaka2Takahiko Sugimoto3Yuka Kobayashi4Taichi Kusakabe5Keisuke Kato6Shogo Hamaguchi7Iyuki Namekata8Hikaru Tanaka9Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Organic Chemistry, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Organic Chemistry, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, JapanThe electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The remaining quiescent preparations had a resting membrane potential less negative than that in the left atria. Blockade of the acetylcholine activated potassium current (IK-ACh) by tertiapin induced a depolarizing shift of the resting membrane potential and automatic electrical activity in the pulmonary vein, but not in the atria. The tertiapin-induced electrical activity, as well as the spontaneous activity, was inhibited by the application of carbachol or by chelation of intracellular Ca2+ by BAPTA. The isolated pulmonary vein cardiomyocytes had an IK-ACh density similar to that of the atrial cardiomyocytes, but a lower density of the inwardly-rectifying potassium current (IK1). Spontaneous Ca2+ transients were observed in about 30% of the isolated pulmonary vein cardiomyocytes, but not in atrial cardiomyocytes. The Ca2+ transients in the pulmonary vein cardiomyocytes were induced by tertiapin and inhibited by carbachol. These results indicate that the pulmonary vein cardiomyocytes have a reduced density of the inwardly-rectifying potassium current, which plays a permissive role in their intracellular Ca2+-dependent automaticity.http://www.sciencedirect.com/science/article/pii/S1347861317300427Pulmonary vein myocardiumPotassium channelAutomaticityResting membrane potentialIntracellular Ca2+ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yayoi Tsuneoka Masahiko Irie Yusuke Tanaka Takahiko Sugimoto Yuka Kobayashi Taichi Kusakabe Keisuke Kato Shogo Hamaguchi Iyuki Namekata Hikaru Tanaka |
spellingShingle |
Yayoi Tsuneoka Masahiko Irie Yusuke Tanaka Takahiko Sugimoto Yuka Kobayashi Taichi Kusakabe Keisuke Kato Shogo Hamaguchi Iyuki Namekata Hikaru Tanaka Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium Journal of Pharmacological Sciences Pulmonary vein myocardium Potassium channel Automaticity Resting membrane potential Intracellular Ca2+ |
author_facet |
Yayoi Tsuneoka Masahiko Irie Yusuke Tanaka Takahiko Sugimoto Yuka Kobayashi Taichi Kusakabe Keisuke Kato Shogo Hamaguchi Iyuki Namekata Hikaru Tanaka |
author_sort |
Yayoi Tsuneoka |
title |
Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
title_short |
Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
title_full |
Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
title_fullStr |
Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
title_full_unstemmed |
Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
title_sort |
permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2017-04-01 |
description |
The electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The remaining quiescent preparations had a resting membrane potential less negative than that in the left atria. Blockade of the acetylcholine activated potassium current (IK-ACh) by tertiapin induced a depolarizing shift of the resting membrane potential and automatic electrical activity in the pulmonary vein, but not in the atria. The tertiapin-induced electrical activity, as well as the spontaneous activity, was inhibited by the application of carbachol or by chelation of intracellular Ca2+ by BAPTA. The isolated pulmonary vein cardiomyocytes had an IK-ACh density similar to that of the atrial cardiomyocytes, but a lower density of the inwardly-rectifying potassium current (IK1). Spontaneous Ca2+ transients were observed in about 30% of the isolated pulmonary vein cardiomyocytes, but not in atrial cardiomyocytes. The Ca2+ transients in the pulmonary vein cardiomyocytes were induced by tertiapin and inhibited by carbachol. These results indicate that the pulmonary vein cardiomyocytes have a reduced density of the inwardly-rectifying potassium current, which plays a permissive role in their intracellular Ca2+-dependent automaticity. |
topic |
Pulmonary vein myocardium Potassium channel Automaticity Resting membrane potential Intracellular Ca2+ |
url |
http://www.sciencedirect.com/science/article/pii/S1347861317300427 |
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