Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P AOX1 -driven recombinant producer strains with different target gene dosage

Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P AOX1 -driven recombinant producer strains with different target gene dosage

Abstract Background The P AOX1 -based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of...

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Bibliographic Details
Main Authors: Javier Garrigós-Martínez, Miguel Angel Nieto-Taype, Arnau Gasset-Franch, José Luis Montesinos-Seguí, Xavier Garcia-Ortega, Francisco Valero
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Microbial Cell Factories
Subjects:
AOX1 promoter
Heterologous gene dosage
Transcription analysis
Pichia pastoris
Gene expression/regulation
MIT1
Online Access:http://link.springer.com/article/10.1186/s12934-019-1240-8

Internet

http://link.springer.com/article/10.1186/s12934-019-1240-8

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