Removal of between-run variation in a multi-plate qPCR experiment

Quantitative PCR (qPCR) is the method of choice in gene expression analysis. However, the number of groups or treatments, target genes and technical replicates quickly exceeds the capacity of a single run on a qPCR machine and the measurements have to be spread over more than 1 plate. Such multi-pla...

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Bibliographic Details
Main Authors: Jan M. Ruijter, Adrián Ruiz Villalba, Jan Hellemans, Andreas Untergasser, Maurice J.B. van den Hoff
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Biomolecular Detection and Quantification
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753515300012