Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis

Abstract Background Spirocerca lupi, the dog oesophageal nematode, causes a potentially fatal disease in domestic dogs, and is currently clinically diagnosed by coproscopy and oesophagoscopy. To date, a single molecular method, a semi-nested PCR, targeting the cox1 gene, has been developed to aid in...

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Bibliographic Details
Main Authors: Alicia Rojas, Gilad Segev, Alex Markovics, Itamar Aroch, Gad Baneth
Format: Article
Language:English
Published: BMC 2017-09-01
Series:Parasites & Vectors
Subjects:
PCR
18S
Online Access:http://link.springer.com/article/10.1186/s13071-017-2374-3