qPCR data analysis: Better results through iconoclasm

The standard approach for quantitative estimation of genetic materials with qPCR is calibration with known concentrations for the target substance, in which estimates of the quantification cycle (Cq) are fitted to a straight-line function of log(N0), where N0 is the initial number of target molecule...

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Bibliographic Details
Main Authors: Joel Tellinghuisen, Andrej-Nikolai Spiess
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Biomolecular Detection and Quantification
Online Access:http://www.sciencedirect.com/science/article/pii/S221475351830010X