qPCR data analysis: Better results through iconoclasm
The standard approach for quantitative estimation of genetic materials with qPCR is calibration with known concentrations for the target substance, in which estimates of the quantification cycle (Cq) are fitted to a straight-line function of log(N0), where N0 is the initial number of target molecule...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2019-03-01
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Series: | Biomolecular Detection and Quantification |
Online Access: | http://www.sciencedirect.com/science/article/pii/S221475351830010X |