Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes.

Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes.

Next-generation sequencing plays a central role in the characterization and quantification of transcriptomes. Although numerous metrics are purported to quantify the quality of RNA, there have been no large-scale empirical evaluations of the major determinants of sequencing success. We used a combin...

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Bibliographic Details
Main Authors: Marc T J Johnson, Eric J Carpenter, Zhijian Tian, Richard Bruskiewich, Jason N Burris, Charlotte T Carrigan, Mark W Chase, Neil D Clarke, Sarah Covshoff, Claude W Depamphilis, Patrick P Edger, Falicia Goh, Sean Graham, Stephan Greiner, Julian M Hibberd, Ingrid Jordon-Thaden, Toni M Kutchan, James Leebens-Mack, Michael Melkonian, Nicholas Miles, Henrietta Myburg, Jordan Patterson, J Chris Pires, Paula Ralph, Megan Rolf, Rowan F Sage, Douglas Soltis, Pamela Soltis, Dennis Stevenson, C Neal Stewart, Barbara Surek, Christina J M Thomsen, Juan Carlos Villarreal, Xiaolei Wu, Yong Zhang, Michael K Deyholos, Gane Ka-Shu Wong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3504007?pdf=render

Internet

http://europepmc.org/articles/PMC3504007?pdf=render

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