Single tracer-based protocol for broad-spectrum kinase profiling in live cells with NanoBRET

Summary: This protocol is used to profile the engagement of kinase inhibitors across nearly 200 kinases in a live-cell context. This protocol utilizes one single kinase tracer (NanoBRET(TM) Tracer K10) that operates quantitatively at four different concentrations. Minimizing the number of tracers of...

Full description

Bibliographic Details
Main Authors: Matthew B. Robers, Jennifer M. Wilkinson, James D. Vasta, Lena M. Berger, Benedict-Tilman Berger, Stefan Knapp
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005281