Single tracer-based protocol for broad-spectrum kinase profiling in live cells with NanoBRET

Single tracer-based protocol for broad-spectrum kinase profiling in live cells with NanoBRET

Summary: This protocol is used to profile the engagement of kinase inhibitors across nearly 200 kinases in a live-cell context. This protocol utilizes one single kinase tracer (NanoBRET(TM) Tracer K10) that operates quantitatively at four different concentrations. Minimizing the number of tracers of...

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Bibliographic Details
Main Authors: Matthew B. Robers, Jennifer M. Wilkinson, James D. Vasta, Lena M. Berger, Benedict-Tilman Berger, Stefan Knapp
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
cell biology
cell-based assays
high-throughput screening
molecular biology
molecular/chemical probes
biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005281

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http://www.sciencedirect.com/science/article/pii/S2666166721005281

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