Efficient targeted integration directed by short homology in zebrafish and mammalian cells

Efficient targeted integration directed by short homology in zebrafish and mammalian cells

Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses sh...

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Bibliographic Details
Main Authors: Wesley A Wierson, Jordan M Welker, Maira P Almeida, Carla M Mann, Dennis A Webster, Melanie E Torrie, Trevor J Weiss, Sekhar Kambakam, Macy K Vollbrecht, Merrina Lan, Kenna C McKeighan, Jacklyn Levey, Zhitao Ming, Alec Wehmeier, Christopher S Mikelson, Jeffrey A Haltom, Kristen M Kwan, Chi-Bin Chien, Darius Balciunas, Stephen C Ekker, Karl J Clark, Beau R Webber, Branden S Moriarity, Stacy L Solin, Daniel F Carlson, Drena L Dobbs, Maura McGrail, Jeffrey Essner
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-05-01
Series:eLife
Subjects:
CRISPR/Cas9
knock-in
targeted integration
human
pig fibroblasts
end joining
Online Access:https://elifesciences.org/articles/53968

Internet

https://elifesciences.org/articles/53968

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