An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays.
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in compariso...
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doaj-755bce092b854995b7e026234e499a722020-11-25T01:51:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7908310.1371/journal.pone.0079083An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays.Romana HöftbergerLidia SabaterRomain MarignierFahmy Aboul-EneinRaphaël Bernard-ValnetHelmut RauschkaAnne RuizYolanda BlancoFrancesc GrausJosep DalmauAlbert SaizCell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection.http://europepmc.org/articles/PMC3817095?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Romana Höftberger Lidia Sabater Romain Marignier Fahmy Aboul-Enein Raphaël Bernard-Valnet Helmut Rauschka Anne Ruiz Yolanda Blanco Francesc Graus Josep Dalmau Albert Saiz |
spellingShingle |
Romana Höftberger Lidia Sabater Romain Marignier Fahmy Aboul-Enein Raphaël Bernard-Valnet Helmut Rauschka Anne Ruiz Yolanda Blanco Francesc Graus Josep Dalmau Albert Saiz An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. PLoS ONE |
author_facet |
Romana Höftberger Lidia Sabater Romain Marignier Fahmy Aboul-Enein Raphaël Bernard-Valnet Helmut Rauschka Anne Ruiz Yolanda Blanco Francesc Graus Josep Dalmau Albert Saiz |
author_sort |
Romana Höftberger |
title |
An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. |
title_short |
An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. |
title_full |
An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. |
title_fullStr |
An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. |
title_full_unstemmed |
An optimized immunohistochemistry technique improves NMO-IgG detection: study comparison with cell-based assays. |
title_sort |
optimized immunohistochemistry technique improves nmo-igg detection: study comparison with cell-based assays. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays. We describe the sensitivity of an optimized immunohistochemistry (IHC-o) to detect NMO-IgG/aquaporin-4-antibody in comparison with that of two CBA: an in-house (CBA-ih) and a commercial (CBA-c) assay (Euroimmun, Germany). Coded serum from 103 patients with definite NMO and 122 inflammatory controls were studied by IHC-o, CBA-ih, and CBA-c. IHC-o used the same protocol described to detect antibodies against cell surface antigens. CBA-ih used live cells transfected with the aquaporin-4-M23-isoform. The sensitivity of the IHC-o was 74.8% (95% confidence interval [CI] 65-83) and was similar to that of the CBA-ih 75.7% (95% CI 66-84) and the CBA-c 73.8% (95% CI 64-82). The specificity of the three assays was 100% (95% CI 97-100). Interassay concordance was high, 100 of 103 samples were coincident in all techniques. The optimized immunohistochemistry proves to be as sensitive and specific as the cell-based assays. This assay extends the available tools for NMO-IgG/aquaporin-4-antibody detection. |
url |
http://europepmc.org/articles/PMC3817095?pdf=render |
work_keys_str_mv |
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