An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis of protein function. However, Cas9-assisted epitope tagging in primary mammalian cell cultures is often inefficient and reliant on plasmid-based selection strategies. Here, w...

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Bibliographic Details
Main Authors: Pooran Singh Dewari, Benjamin Southgate, Katrina Mccarten, German Monogarov, Eoghan O'Duibhir, Niall Quinn, Ashley Tyrer, Marie-Christin Leitner, Colin Plumb, Maria Kalantzaki, Carla Blin, Rebecca Finch, Raul Bardini Bressan, Gillian Morrison, Ashley M Jacobi, Mark A Behlke, Alex von Kriegsheim, Simon Tomlinson, Jeroen Krijgsveld, Steven M Pollard
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-04-01
Series:eLife
Subjects:
neural stem cell
CRISPR
epitope tagging
stem cells
genome editing
transcription factors
Online Access:https://elifesciences.org/articles/35069

Internet

https://elifesciences.org/articles/35069

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