Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.

Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.

Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonst...

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Bibliographic Details
Main Authors: Michael J Moser, Robert A DiFrancesco, Krishne Gowda, Audrey J Klingele, Darby R Sugar, Stacy Stocki, David A Mead, Thomas W Schoenfeld
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3366922?pdf=render

Internet

http://europepmc.org/articles/PMC3366922?pdf=render

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