Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.
Viral metagenomic libraries are a promising but previously untapped source of new reagent enzymes. Deep sequencing and functional screening of viral metagenomic DNA from a near-boiling thermal pool identified clones expressing thermostable DNA polymerase (Pol) activity. Among these, 3173 Pol demonst...
Main Authors: | Michael J Moser, Robert A DiFrancesco, Krishne Gowda, Audrey J Klingele, Darby R Sugar, Stacy Stocki, David A Mead, Thomas W Schoenfeld |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3366922?pdf=render |
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