Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome

Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome

Post-translational modifications (PTMs) play a key role in regulating protein function, yet their identification is technically demanding. Here, we present a straightforward workflow to systematically identify post-translationally modified proteins based on two-dimensional gel electrophoresis. Upon...

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Bibliographic Details
Main Authors: Kathrin Kusch, Marina Uecker, Thomas Liepold, Wiebke Möbius, Christian Hoffmann, Heinz Neumann, Hauke B. Werner, Olaf Jahn
Format: Article
Language:English
Published: MDPI AG 2017-01-01
Series:Proteomes
Subjects:
post-translational modification (PTM)
2D gel electrophoresis (2DE) blot
myelin
acetylome
SERPA
immunoproteomics
tubulin acetylation
cyclic nucleotide phosphodiesterase (CNP)
septin 8 (SEPT8)
isoelectric focusing (IEF)
immunoblot
cryo immuno-electron microscopy (IEM)
Online Access:http://www.mdpi.com/2227-7382/5/1/3

Internet

http://www.mdpi.com/2227-7382/5/1/3

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