Streamlining DNA barcoding protocols: automated DNA extraction and a new cox1 primer in arachnid systematics.

BACKGROUND: DNA barcoding is a popular tool in taxonomic and phylogenetic studies, but for most animal lineages protocols for obtaining the barcoding sequences--mitochondrial cytochrome C oxidase subunit I (cox1 AKA CO1)--are not standardized. Our aim was to explore an optimal strategy for arachnids...

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Bibliographic Details
Main Authors: Nina Vidergar, Nataša Toplak, Matjaž Kuntner
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4240537?pdf=render