Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters

Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters

To monitor nitrate and peptide transport activity in vivo, we converted the dual-affinity nitrate transceptor CHL1/NRT1.1/NPF6.3 and four related oligopeptide transporters PTR1, 2, 4, and 5 into fluorescence activity sensors (NiTrac1, PepTrac). Substrate addition to yeast expressing transporter fusi...

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Bibliographic Details
Main Authors: Cheng-Hsun Ho, Wolf B Frommer
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-03-01
Series:eLife
Subjects:
biosensor
FRET
fluorescence
transport
nitrate
oligopeptide
Online Access:https://elifesciences.org/articles/01917

Internet

https://elifesciences.org/articles/01917

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