Engineering processive DNA polymerases with maximum benefit and minimum cost

DNA polymerases need to be engineered to achieve optimal performance for biotechnological applications, which often require high fidelity replication when using modified nucleotides and when replicating difficult DNA sequences. These tasks are achieved for the bacteriophage T4 DNA polymerase by rep...

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Bibliographic Details
Main Authors: Linda J. Reha-Krantz, Sandra eWoodgate, Myron F. Goodman
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-08-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00380/full