Quantification of DNA Extracted from Formalin Fixed Paraffin-Embeded Tissue Comparison of Three Techniques: Effect on PCR Efficiency

Quantification of DNA Extracted from Formalin Fixed Paraffin-Embeded Tissue Comparison of Three Techniques: Effect on PCR Efficiency

Introduction: Mutation detection from Formalin Fixed ParaffinEmbedding (FFPE) tissue in molecular lab became a necessary tool for defining potential targeted drug. Accurate quantification of DNA extracted from FFPE tissue is necessary for downstream applications like Polymerase Chain Reaction (PC...

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Bibliographic Details
Main Authors: Dushyant Kumar, Manoj Kumar Panigrahi, Moushumi Suryavanshi, Anurag Mehta, Kandarpa Kumar Saikia
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2016-09-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
fluorometer
qpcr
sequencing
spectrophotometer
Online Access:https://jcdr.net/articles/PDF/8407/19383_CE(RA1)_F(T)_PF1(ASAK)_PFA(AK)_PF2(PAG).pdf

Internet

https://jcdr.net/articles/PDF/8407/19383_CE(RA1)_F(T)_PF1(ASAK)_PFA(AK)_PF2(PAG).pdf

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