ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery

<p>Abstract</p> <p>Background</p> <p>In some genomic applications it is necessary to design large numbers of PCR primers in exons flanking one or several introns on the basis of orthologous gene sequences in related species. The primer pairs designed by this target gene...

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Bibliographic Details
Main Authors: Gu Yong Q, Huo Naxin, You Frank M, Lazo Gerard R, Dvorak Jan, Anderson Olin D
Format: Article
Language:English
Published: BMC 2009-10-01
Series:BMC Bioinformatics
Online Access:http://www.biomedcentral.com/1471-2105/10/331