The structure of a novel thermophilic esterase from the Planctomycetes species, Thermogutta terrifontis reveals an open active site due to a minimal ‘cap’ domain.

The structure of a novel thermophilic esterase from the Planctomycetes species, Thermogutta terrifontis reveals an open active site due to a minimal ‘cap’ domain.

A carboxyl esterase (TtEst2) has been identified in a novel thermophilic bacterium, Thermogutta terrifontis from the phylum Planctomycetes and has been cloned and over-expressed in Escherichia coli. The enzyme has been characterised biochemically and shown to have activity towards small p-nitropheny...

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Bibliographic Details
Main Authors: Jennifer Ann Littlechild, Christopher eSayer, Michail N Isupov, Zalan eSzabo, Colin eIngham
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-11-01
Series:Frontiers in Microbiology
Subjects:
planctomycetes
X-ray structure
carboxyl esterase
Thermophilic enzymes
Biocatalysis.
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01294/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01294/full

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