Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes
Studies in animal models have shown that skin tissue-resident memory T (TRM) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin TRM cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinator...
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2021-03-01
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doaj-7750cbe1eed14c0c962b1a06bb7d1fe02021-03-22T05:02:41ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-03-011210.3389/fimmu.2021.624013624013Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T LymphocytesWeijie Du0Daniel Lenz1Ralf Köhler2Erping Zhang3Carla Cendon4Jinchan Li5Mona Massoud6Joachim Wachtlin7Joachim Wachtlin8Juliane Bodo9Anja E. Hauser10Anja E. Hauser11Andreas Radbruch12Jun Dong13Cell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyCentral Lab for Microscopy, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanySankt Gertrauden Krankenhaus, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanySankt Gertrauden Krankenhaus, Berlin, GermanyMedizinische Hochschule Brandenburg, Neurrupin, GermanyPlastische und Ästhetische Chirurgie, Berlin, GermanyCentral Lab for Microscopy, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyImmune Dynamics, Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyCell Biology, Deutsches Rheuma-Forschungszentrum Berlin, Institute of the Leibniz Association, Berlin, GermanyStudies in animal models have shown that skin tissue-resident memory T (TRM) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin TRM cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinatorial strategy-the 6-h collagenase IV digestion and gentle tissue dissociation – for rapid and efficient isolation of skin TRM cells with skin tissue-specific immune features. In comparison with paired blood circulating memory T cells, these ex vivo isolated skin T cells express typical TRM cell markers and display higher polyfunctional properties. Moreover, these isolated cells can also be assessed for longer periods of time in ex vivo cultures. Thus, the optimized isolation protocol provides a valuable tool for further understanding of human skin TRM cells, especially for direct comparison with peripheral blood T cells at the same sample collection time.https://www.frontiersin.org/articles/10.3389/fimmu.2021.624013/fullhuman skintissue-resident memory T cellsyieldepitopecollagenase IVgentle tissue dissociation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weijie Du Daniel Lenz Ralf Köhler Erping Zhang Carla Cendon Jinchan Li Mona Massoud Joachim Wachtlin Joachim Wachtlin Juliane Bodo Anja E. Hauser Anja E. Hauser Andreas Radbruch Jun Dong |
spellingShingle |
Weijie Du Daniel Lenz Ralf Köhler Erping Zhang Carla Cendon Jinchan Li Mona Massoud Joachim Wachtlin Joachim Wachtlin Juliane Bodo Anja E. Hauser Anja E. Hauser Andreas Radbruch Jun Dong Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes Frontiers in Immunology human skin tissue-resident memory T cells yield epitope collagenase IV gentle tissue dissociation |
author_facet |
Weijie Du Daniel Lenz Ralf Köhler Erping Zhang Carla Cendon Jinchan Li Mona Massoud Joachim Wachtlin Joachim Wachtlin Juliane Bodo Anja E. Hauser Anja E. Hauser Andreas Radbruch Jun Dong |
author_sort |
Weijie Du |
title |
Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes |
title_short |
Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes |
title_full |
Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes |
title_fullStr |
Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes |
title_full_unstemmed |
Rapid Isolation of Functional ex vivo Human Skin Tissue-Resident Memory T Lymphocytes |
title_sort |
rapid isolation of functional ex vivo human skin tissue-resident memory t lymphocytes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-03-01 |
description |
Studies in animal models have shown that skin tissue-resident memory T (TRM) cells provide enhanced and immediate effector function at the site of infection. However, analyses of skin TRM cells in humans have been hindered by the lack of an optimized isolation protocol. Here, we present a combinatorial strategy-the 6-h collagenase IV digestion and gentle tissue dissociation – for rapid and efficient isolation of skin TRM cells with skin tissue-specific immune features. In comparison with paired blood circulating memory T cells, these ex vivo isolated skin T cells express typical TRM cell markers and display higher polyfunctional properties. Moreover, these isolated cells can also be assessed for longer periods of time in ex vivo cultures. Thus, the optimized isolation protocol provides a valuable tool for further understanding of human skin TRM cells, especially for direct comparison with peripheral blood T cells at the same sample collection time. |
topic |
human skin tissue-resident memory T cells yield epitope collagenase IV gentle tissue dissociation |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.624013/full |
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