Tuning the Cell-Free Protein Synthesis System for Biomanufacturing of Monomeric Human Filaggrin

The modern cell-free protein synthesis (CFPS) system is expanding the opportunity of cell-free biomanufacturing as a versatile platform for synthesizing various therapeutic proteins. However, synthesizing human protein in the bacterial CFPS system remains challenging due to the low expression level,...

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Bibliographic Details
Main Authors: Jeehye Kim, Caroline E. Copeland, Kosuke Seki, Bastian Vögeli, Yong-Chan Kwon
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.590341/full