Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Abstract Background The International Mouse Phenotyping Consortium is generating null allele mice for every protein-coding gene in the genome and characterizing these mice to identify gene–phenotype associations. While CRISPR/Cas9-mediated null allele production in mice is highly efficient, generati...

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Bibliographic Details
Main Authors: Denise G. Lanza, Angelina Gaspero, Isabel Lorenzo, Lan Liao, Ping Zheng, Ying Wang, Yu Deng, Chonghui Cheng, Chuansheng Zhang, John R. Seavitt, Francesco J. DeMayo, Jianming Xu, Mary E. Dickinson, Arthur L. Beaudet, Jason D. Heaney
Format: Article
Language:English
Published: BMC 2018-06-01
Series:BMC Biology
Subjects:
CRISPR/Cas9
Conditional null allele
High-throughput production
Genome editing
Single-stranded DNA oligonucleotides
Homology directed repair
Online Access:http://link.springer.com/article/10.1186/s12915-018-0529-0

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http://link.springer.com/article/10.1186/s12915-018-0529-0

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