CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.
The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We examined the expression of CD57 on T cells...
Main Authors: | , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3585296?pdf=render |
id |
doaj-77a2d7b772234c8c9042e76f1450891f |
---|---|
record_format |
Article |
spelling |
doaj-77a2d7b772234c8c9042e76f1450891f2020-11-25T00:23:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5214410.1371/journal.pone.0052144CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.Mariana D BatistaCamilla TincatiJeffrey M MilushEmily L HoLishomwa C NdhlovuVanessa A YorkEsper G KallasJorge KalilSheila M KeatingPhilip J NorrisDavid ChangPatrick UnemoriKieron S LeslieToby MaurerWilson LiaoDouglas F NixonThe immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.http://europepmc.org/articles/PMC3585296?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariana D Batista Camilla Tincati Jeffrey M Milush Emily L Ho Lishomwa C Ndhlovu Vanessa A York Esper G Kallas Jorge Kalil Sheila M Keating Philip J Norris David Chang Patrick Unemori Kieron S Leslie Toby Maurer Wilson Liao Douglas F Nixon |
spellingShingle |
Mariana D Batista Camilla Tincati Jeffrey M Milush Emily L Ho Lishomwa C Ndhlovu Vanessa A York Esper G Kallas Jorge Kalil Sheila M Keating Philip J Norris David Chang Patrick Unemori Kieron S Leslie Toby Maurer Wilson Liao Douglas F Nixon CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. PLoS ONE |
author_facet |
Mariana D Batista Camilla Tincati Jeffrey M Milush Emily L Ho Lishomwa C Ndhlovu Vanessa A York Esper G Kallas Jorge Kalil Sheila M Keating Philip J Norris David Chang Patrick Unemori Kieron S Leslie Toby Maurer Wilson Liao Douglas F Nixon |
author_sort |
Mariana D Batista |
title |
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. |
title_short |
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. |
title_full |
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. |
title_fullStr |
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. |
title_full_unstemmed |
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis. |
title_sort |
cd57 expression and cytokine production by t cells in lesional and unaffected skin from patients with psoriasis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover. |
url |
http://europepmc.org/articles/PMC3585296?pdf=render |
work_keys_str_mv |
AT marianadbatista cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT camillatincati cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT jeffreymmilush cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT emilylho cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT lishomwacndhlovu cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT vanessaayork cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT espergkallas cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT jorgekalil cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT sheilamkeating cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT philipjnorris cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT davidchang cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT patrickunemori cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT kieronsleslie cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT tobymaurer cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT wilsonliao cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis AT douglasfnixon cd57expressionandcytokineproductionbytcellsinlesionalandunaffectedskinfrompatientswithpsoriasis |
_version_ |
1725357000479473664 |