PhotoGate microscopy to track single molecules in crowded environments
Tracking single molecules in living cells is difficult or impractical owing to their high density. Here the authors introduce a technique of repeatedly photobleaching fluorophores at the boundary of a region of interest, thereby controlling the number of fluorescent particles for observation.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2017-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms13978 |