Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Abstract Objective Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays t...

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Bibliographic Details
Main Authors: Julian Geiger, Rebecca Doelker, Sofia Salö, Thomas Roitsch, Louise T. Dalgaard
Format: Article
Language:English
Published: BMC 2019-10-01
Series:BMC Research Notes
Subjects:
INS-1E
Enzyme assays
96 well format
Aldolase
Hexokinase
Glucose-6-phosphate dehydrogenase
Online Access:http://link.springer.com/article/10.1186/s13104-019-4697-y

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http://link.springer.com/article/10.1186/s13104-019-4697-y

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