A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production

A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production

Pichia pastoris is a non-conventional methylotrophic yeast that is widely used for recombinant protein production, typically by stably integrating the target gene into the genome as part of an expression cassette. However, the comparatively high clonal variability associated with this approach usual...

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Bibliographic Details
Main Authors: Karl Friehs, Jan-Philipp Schwarzhans, Tobias Luttermann, Daniel Wibberg, Anika Winkler, Wolfgang Hübner, Thomas Huser, Jörn Kalinowski
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Microbiology
Subjects:
episomal vectors
mitochondrial DNA migration
non-homologous end joining
recombinant protein production
autonomously replicating sequence consensus sequence
Pichia pastoris
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.00780/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.00780/full

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