A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq

Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision. Results We describe a method for sequencing near full-len...

Full description

Bibliographic Details
Main Authors: Catherine M. Burke, Aaron E. Darling
Format: Article
Language:English
Published: PeerJ Inc. 2016-09-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/2492.pdf