Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system

Abstract Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell. We are interested in creating synthetic genetic circuits that function in a cell-free expression system. This will req...

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Bibliographic Details
Main Authors: Xinying Shi, Ti Wu, Christian M. Cole, Neal K. Devaraj, Simpson Joseph
Format: Article
Language:English
Published: Nature Publishing Group 2018-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-21739-6