Hyperspectral imaging for simultaneous measurements of two FRET biosensors in pancreatic β-cells.

Fluorescent protein (FP) biosensors based on Förster resonance energy transfer (FRET) are commonly used to study molecular processes in living cells. There are FP-FRET biosensors for many cellular molecules, but it remains difficult to perform simultaneous measurements of multiple biosensors. The ov...

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Bibliographic Details
Main Authors: Amicia D Elliott, Noah Bedard, Alessandro Ustione, Michelle A Baird, Michael W Davidson, Tomasz Tkaczyk, David W Piston
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5718502?pdf=render