Expression of an endoglucanase–cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi

Abstract The low secretion levels of cellobiohydrolase I (CBHI) in yeasts are one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this obstacle, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI b...

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Bibliographic Details
Main Authors: Qi Xu, Markus Alahuhta, Hui Wei, Eric P. Knoshaug, Wei Wang, John O. Baker, Todd Vander Wall, Michael E. Himmel, Min Zhang
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Biotechnology for Biofuels
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13068-018-1301-y