Isolation of mouse Kupffer cells for phenotypic and functional studies

Isolation of mouse Kupffer cells for phenotypic and functional studies

Summary: Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted...

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Bibliographic Details
Main Authors: Francesco Andreata, Camille Blériot, Pietro Di Lucia, Giorgia De Simone, Valeria Fumagalli, Xenia Ficht, Cristian Gabriel Beccaria, Mirela Kuka, Florent Ginhoux, Matteo Iannacone
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005372

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http://www.sciencedirect.com/science/article/pii/S2666166721005372

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