Isolation of mouse Kupffer cells for phenotypic and functional studies

Isolation of mouse Kupffer cells for phenotypic and functional studies

Summary: Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted...

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Bibliographic Details
Main Authors: Francesco Andreata, Camille Blériot, Pietro Di Lucia, Giorgia De Simone, Valeria Fumagalli, Xenia Ficht, Cristian Gabriel Beccaria, Mirela Kuka, Florent Ginhoux, Matteo Iannacone
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005372
Description
Summary:Summary: Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations.For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).
ISSN:2666-1667

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