NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions.
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of ampl...
Main Authors: | Andrey Kechin, Viktoria Borobova, Ulyana Boyarskikh, Evgeniy Khrapov, Sergey Subbotin, Maxim Filipenko |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-12-01
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Series: | PLoS Computational Biology |
Online Access: | https://doi.org/10.1371/journal.pcbi.1008468 |
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