Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Abstract Background Metabolic engineering frequently needs genomic integration of many heterologous genes for biosynthetic pathway assembly. Despite great progresses in genome editing for the model microorganism Escherichia coli, the integration of large pathway into genome for stabilized chemical p...

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Bibliographic Details
Main Authors: Buli Su, Dandan Song, Honghui Zhu
Format: Article
Language:English
Published: BMC 2020-05-01
Series:Microbial Cell Factories
Subjects:
Metabolic engineering
Chromosomal integration
Lambda Red
CRISPR-Cas9
Escherichia coli
Online Access:http://link.springer.com/article/10.1186/s12934-020-01360-x

Internet

http://link.springer.com/article/10.1186/s12934-020-01360-x

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