Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone

Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone

A goal for microbial ecophysiological research is to reveal microbial activities in natural environments, including sediments, soils, or infected human tissues. Here, we report the application of the hybridization chain reaction (HCR) v3.0 to quantitatively measure microbial gene expression in situ...

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Bibliographic Details
Main Authors: Peter Jorth, Melanie A. Spero, J. Livingston, Dianne K. Newman
Format: Article
Language:English
Published: American Society for Microbiology 2019-12-01
Series:mBio
Subjects:
hcr
pseudomonas aeruginosa
aggregate
biofilms
gene expression
in situ hybridization
microscopy
Online Access:https://doi.org/10.1128/mBio.02622-19

Internet

https://doi.org/10.1128/mBio.02622-19

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