Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars

Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars

CRISPR/Cas9 has become one of the most promising techniques for genome editing in plants and works very well in poplars with an <i>Agrobacterium</i>-mediated transformation system. We selected twelve genes, including <i>SOC1</i>, <i>FUL</i>, and their paralogous g...

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Bibliographic Details
Main Authors: Tobias Bruegmann, Khira Deecke, Matthias Fladung
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:International Journal of Molecular Sciences
Subjects:
gRNA
homozygous editing
<i>Populus</i>
secondary structure
sequencing
transformation
Online Access:https://www.mdpi.com/1422-0067/20/15/3623

Internet

https://www.mdpi.com/1422-0067/20/15/3623

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